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KMID : 0381120120340060709
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Genes and Genomics
2012 Volume.34 No. 6 p.709 ~ p.713
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Reactive oxygen species-dependent transcriptional regulation of peroxisome proliferator-activated receptor γ coactivator 1α in a human hepatocarcinoma cell line
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Kim Kyung-Hoon
Kim Su-Jung
Cho Nam-Chul
Jung Hyun-Joo
Ahn Ki-Sup
Ryu In-Wang
Lim Chang-Jin
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Abstract
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The present work aimed to examine whether transcriptional regulation of the human PGC-1α gene encoding peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) is subjected to oxidative stress or not. Oxidative stressors, such as superoxide radical-generating menadione (MD) and hydrogen peroxide (H2O2), brought about a diminishment in the PGC-1α mRNA levels of the human hepatocarcinoma HepG2 cells. The PGC-1α mRNA level was enhanced by the treatment with exogenous antioxidants, such as glutathione (GSH) and N-acetylcysteine (NAC). The HepG2 stable transfectants overexpressing the mitochondrial isoform of human glutaredoxin, Grx2a, were assessed to contain the significantly increased PGC-1α mRNA level, compared to the vector control cells. Taken together, transcription of the human PGC-1α gene is negatively regulated by reactive oxygen species (ROS) in a human hepatocarcinoma cell line.
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KEYWORD
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Glutaredoxin, Glutathione, Oxidative stress, PGC-1 α, Reactive oxygen species
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